Long-R3-IGF-1(Lyophilized/NonDiluted) 1mg (Includes Acetic Acid)

Molecular Weight: 9110±2KDa. Loading mass is not less than 10ug
Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pro-Ala-Met.

Appearance: White Freeze-dried powder

HPLC Purity: More than 98.02%

Endotoxin Level: Less than 1.0EU/µg of Long-R3-IGF-I.

Formulation: Long-R3-IGF-I is in lyophilized/non diluted form. 

Freeze-dried powder can be stored at -20 degree C for a year. Reconstituted solution should be kept in the refrigerator at 2-8 degree C and used as early as possible. Avoid multiple freeze-thaw cycles and exposure to frequent for lyophilized powder or reconstituted vials.

Long Arg3 Insulin-like Growth Factor-I (Long-R3-IGF-I) is an 83 amino acid analog of  IGF-I comprising the complete IGF-I sequence with the substitution of an Arg for the Glu at position 3 (hence R3), and a 13 amino acid extension peptide at the N-terminus. Long-R3-IGF-I is significantly more potent than IGF-I in vitro. The enhanced potency is due to the markedly decreased binding of Long-R3-IGF-I to IGF binding proteins which normally inhibit the biological actions of IGFs.

Recombinant Human Long-R3-IGF-I produced in E. coli is a single, non-glycosylated, polypeptide chain containing 83 amino acids and having a molecular mass of 9111 Dalton.

Biological activity: The ED50, calculated by the dose-dependant proliferation of murine BALB\C 3T3 cells is less then 1.0 ng/ml, corresponding to a specific activity of 1.0 x 106 IU/mg. For most in-vitro applications, Long-R3-IGF-I exerts its biological activity in the concentration range of 0.2-20 ng/ml.